Features • Provides the biological background associated with energy metabolism and other metabolic pathways as well as the spectroscopy of potential biomarkers intrinsic in living cells and tissues • Explores recent advances in natural biomarkers, including quantitative, noninvasive optical methods for cellular/tissue metabolism studies • Covers the theoretical and technical aspects of various fluorescence microscopy and spectroscopy methods used to monitor the autofluorescence of natural biomarkers in living cells/tissues • Explains how to use cellular autofluorescence to investigate metabolic activities, mitochondrial anomalies, and biochemical redox reactions in healthy cells/tissues under different external manipulations induced by drugs, diseases, or environmental hazards • Describes the latest laser-based medical diagnostic approaches that address the cellular and biochemical mechanisms of health problems in both quantitative and noninvasive ways Summary From the Lab to Clinica